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Problem removing ISO file

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I've got an ISO file I've been wanting to delete, but it's been stuck in my desktop for a couple of weeks now. Every time I try to delete it, it gives me an error message saying "The action can't be completed because the file is open in System".

I've closed all of my virtual drives on DAEMON Tools, and then uninstalled it. I've already tried to use MoveOnBoot and LockHunter (Unlocker wouldn't open up). Help please!
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Did you try booting to Safe Mode and deleting from there?
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